A stylus removal mechanism (100), used in portable electronic devices, includes a main body (10), having a latching hole (1121) defined therein, and an elastic device (20). The elastic device is fixed at the main body and includes an elastic member (21), a removal member (22), and a connecting pole (25). The elastic member includes two ends (210) respectively engaging with the removal member and the main body. The removal member has a cam (240) and a conducting groove (24) defined around the cam. The conducting groove has a latching portion (241). The connecting pole includes one end (251), received in the latching hole of the main body, and another end (252) movably received in the conducting groove.