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US07588944B2 Method of analyzing C-terminal amino acid sequence of peptide 失效
分析肽的C-末端氨基酸序列的方法

Method of analyzing C-terminal amino acid sequence of peptide
摘要:
An analyte peptide is selectively degraded sequentially by using an alkanoic anhydride (S101). The original peptide and a series of degradation reaction products having peptide in which one or more C-terminal-sided amino acids are deleted, are subjected to a certain posttreatment (S102). The molecular weight of the reaction products is measured by mass spectrometry (S103). And, the amino acid sequence of the original peptide from C-terminal is determined, based on the molecular weight obtained by mass spectrometry (S104).
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