Suture separation and organization devices for use in a tissue graft tensioning system. The inventive devices help to separate and organize a plurality of suture strands that extend away from a bone tunnel of a patient's leg during joint repair surgery (e.g., while repairing or replacing the anterior cruciate ligament (“ACL”)). The suture separation and organization device may comprise one or more elongate bodies, or a one-piece body, comprising a plurality of suture retention recesses or protrusions disposed on a perimeter of a body. The suture separation and organization device also includes recesses or passages adapted to receive corresponding posts of a graft tensioning device. In this way, the suture separation and organization device can be removably attached to the graft tensioning device.