发明授权
US07709666B2 Transgenic plants containing altered levels of sterol compounds and tocopherols
有权
含有改变水平的固醇化合物和生育酚的转基因植物
- 专利标题: Transgenic plants containing altered levels of sterol compounds and tocopherols
- 专利标题(中): 含有改变水平的固醇化合物和生育酚的转基因植物
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申请号: US10647517申请日: 2004-01-16
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公开(公告)号: US07709666B2公开(公告)日: 2010-05-04
- 发明人: Mylavarapu Venkatramesh , David R. Corbin , B. Ganesh Bhat , Sekhar S. Boddupalli , Robert J. Grebenok , Ganesh M. Kishore , Kathryn D. Lardizabal , Michael W. Lassner , Shaukat H. Rangwala , Balasulojini Karunanandaa
- 申请人: Mylavarapu Venkatramesh , David R. Corbin , B. Ganesh Bhat , Sekhar S. Boddupalli , Robert J. Grebenok , Ganesh M. Kishore , Kathryn D. Lardizabal , Michael W. Lassner , Shaukat H. Rangwala , Balasulojini Karunanandaa
- 申请人地址: US MO St. Louis
- 专利权人: Monsanto Technology LLC
- 当前专利权人: Monsanto Technology LLC
- 当前专利权人地址: US MO St. Louis
- 代理机构: Arnold & Porter LLP
- 代理商 Chunping Li
- 主分类号: C07C59/147
- IPC分类号: C07C59/147
摘要:
Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and α-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5α-reductases are also disclosed.