发明授权
US07709700B2 Method for improving plant transformation efficiency by adding copper ion
有权
通过添加铜离子提高植物转化效率的方法
- 专利标题: Method for improving plant transformation efficiency by adding copper ion
- 专利标题(中): 通过添加铜离子提高植物转化效率的方法
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申请号: US10567965申请日: 2004-08-12
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公开(公告)号: US07709700B2公开(公告)日: 2010-05-04
- 发明人: Yuji Ishida
- 申请人: Yuji Ishida
- 申请人地址: JP Tokyo
- 专利权人: Japan Tobacco Inc.
- 当前专利权人: Japan Tobacco Inc.
- 当前专利权人地址: JP Tokyo
- 代理机构: Birch, Stewart, Kolasch & Birch, LLP
- 优先权: JP2003-293062 20030813
- 国际申请: PCT/JP2004/011599 WO 20040812
- 国际公布: WO2005/017152 WO 20050224
- 主分类号: C12N15/84
- IPC分类号: C12N15/84 ; A01H4/00
摘要:
The present invention provides a method for Agrobacterium-mediated gene introduction into a plant material, comprising: 1) treating the plant material, and then 2) infecting the plant material with an Agrobacterium, characterized in that a medium enriched in a metal salt containing copper ion is used in step 1) and/or 2). The present invention also provides a process for preparing a transformed plant characterized in that the gene introduction method of the present invention is used.
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