发明授权
US07723081B1 Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same
有权
含有天冬氨酸 - 半醛脱氢酶，磷酸烯醇丙酮酸羧化酶和转氢酶在埃希氏菌中产生L-赖氨酸的细菌及其使用方法
- 专利标题： Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same
- 专利标题（中）： 含有天冬氨酸 - 半醛脱氢酶，磷酸烯醇丙酮酸羧化酶和转氢酶在埃希氏菌中产生L-赖氨酸的细菌及其使用方法
-
申请号： US10149450申请日： 2000-01-21
-
公开(公告)号： US07723081B1公开(公告)日： 2010-05-25
- 发明人: Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
- 申请人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
- 申请人地址： JP Tokyo
- 专利权人： Ajinomoto Co., Inc.
- 当前专利权人： Ajinomoto Co., Inc.
- 当前专利权人地址： JP Tokyo
- 代理机构： Cermak Kenealy Vaidya & Nakajima LLP
- 代理商 Shelly Guest Cermak
- 国际申请： PCT/JP00/00298 WO 20000121
- 国际公布： WO01/53459 WO 20010726
- 主分类号： C12P13/08
- IPC分类号： C12P13/08
摘要：
An Escherichia bacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.