发明授权
US07723568B2 Engineered miRNA precursors for suppressing target gene expression in plants
有权
用于抑制植物中靶基因表达的工程miRNA前体
- 专利标题: Engineered miRNA precursors for suppressing target gene expression in plants
- 专利标题(中): 用于抑制植物中靶基因表达的工程miRNA前体
-
申请号: US11545106申请日: 2006-10-10
-
公开(公告)号: US07723568B2公开(公告)日: 2010-05-25
- 发明人: Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
- 申请人: Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
- 申请人地址: US MO Saint Louis
- 专利权人: Monsanto Technology LLC
- 当前专利权人: Monsanto Technology LLC
- 当前专利权人地址: US MO Saint Louis
- 代理商 Ritesh Agroval; Thomas J. La Rosa; Ying-Hong Liu
- 主分类号: C12N15/82
- IPC分类号: C12N15/82 ; C12N5/10 ; A01H5/10 ; A01H5/00
摘要:
The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.