A method and circuit for implementing an eFuse sense amplifier, and a design structure on which the subject circuit resides are provided. A sensing circuit includes a pair of cross-coupled inverters, each formed by a pair of series connected P-channel field effect transistors (PFETs) and an N-channel field effect transistor (NFET). A first pull-up resistor is coupled between a positive voltage supply rail and a first sensing node of the sensing circuit. A second pull-up resistor is coupled between a positive voltage supply rail and a second sensing node of the sensing circuit. A first bitline is coupled to the first sensing node of the sensing circuit and a second bitline coupled to the second sensing node of the sensing circuit. One of a respective reference resistor and a respective eFuse cell is selectively coupled to the first bitline and the second bitline.