Invention Grant
- Patent Title: Process for the purification of antibodies
- Patent Title (中): 抗体纯化方法
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Application No.: US10589717Application Date: 2005-02-25
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Publication No.: US07750129B2Publication Date: 2010-07-06
- Inventor: Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
- Applicant: Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
- Applicant Address: SE Uppsala
- Assignee: GE Healthcare Bio-Sciences AB
- Current Assignee: GE Healthcare Bio-Sciences AB
- Current Assignee Address: SE Uppsala
- Agent Yonggang Ji
- Priority: SE0400501 20040227; SE0402558 20041021
- International Application: PCT/SE2005/000293 WO 20050225
- International Announcement: WO2005/082483 WO 20050909
- Main IPC: C07K1/18
- IPC: C07K1/18 ; C07K1/22

Abstract:
The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
Public/Granted literature
- US20070167613A1 Process for the purification of antibodies Public/Granted day:2007-07-19
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