发明授权
- 专利标题： Identification of high affinity molecules by limited dilution screening
- 专利标题（中）： 通过有限稀释筛选鉴定高亲和力分子
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申请号： US10309421申请日： 2002-12-02
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公开(公告)号： US07754433B2公开(公告)日： 2010-07-13
- 发明人: John Babcook , Xiao-Chi Jia , Jaspal Singh Kang , Li Zhang
- 申请人： John Babcook , Xiao-Chi Jia , Jaspal Singh Kang , Li Zhang
- 申请人地址： US CA Fremont
- 专利权人： Amgen Fremont Inc.
- 当前专利权人： Amgen Fremont Inc.
- 当前专利权人地址： US CA Fremont
- 代理机构： Knobbe Martens Olson & Bear LLP
- 主分类号： G01N33/53
- IPC分类号： G01N33/53
摘要：
Methods are disclosed for determining the relative binding affinities of molecules for their binding partner. One type of molecule is an antibody, which has an antigen binding partner. Antibodies are tested for binding against limited dilutions of antigen. Antibodies that bind to the most dilute antigen concentrations are determined to have a higher relative binding affinity for the antigen than antibodies that only bind to more concentrated antigen preparations.