Provided are an apparatus and method for controlling a data strobe signal. The apparatus includes a period measurement unit measuring a period of an input clock signal or data strobe signal; a controller determining a read delay time, a setup margin delay time, and a hold margin delay time of the data strobe signal on the basis of the period; a delay circuit unit outputting signals generated by delaying the data strobe signal by the read delay time, the setup margin delay time, and the hold margin delay time; a flip-flop unit latching and outputting input data by using the signals output from the delay circuit unit; and a comparator comparing outputs from the flip-flop unit and feeding a result of the comparison back to the controller. Accordingly, it is possible to stably read data recorded in a memory.