It is proposed to provide a printing die clamp which can clamp a printing die by rotating the take-up shaft without rotating the printing cylinder. A printing die clamp is proposed for clamping a printing die 1 wrapped around an outer periphery of a printing cylinder 2 of a printing press against the outer periphery of the printing cylinder 2 by engaging one end of the printing die 1 with the printing cylinder 2 through an engaging means 18 provided on the printing cylinder 2, engaging the other end of the printing die 1 with a take-up shaft 3 rotatably mounted in a receiving groove 19 formed in the outer periphery of the printing cylinder 2, and rotating the take-up shaft 3. The printing cylinder 2 has a tubular printing cylinder shaft 9 in which a control shaft 5 is inserted so as to be rotatable relative to the printing cylinder shaft 9. The printing die clamp further includes gears 21, 22 and 23 for transmitting the rotation of the control shaft 5 to the take-up shaft 3, a die clamp motor 4 for rotating the control shaft 5, and a first clutch 6 provided in a torque transmission path through which the rotation of the die clamp motor 4 is transmitted to the control shaft 5 for selectively transmitting the rotation of the die clamp motor 4 to the control shaft 5.