A drawer interlock mechanism includes at least two drawers, a fixture, a linking frame, locking members and guiding members. The drawers may be pulled in and out. The drawers are lined straight with each other. The fixture is disposed at one side the drawers, and is formed with slots corresponding in position and in number to the guiding members. The linking frame is disposed on the fixture. Each guiding member is secured to the side of each drawer. When one of the drawers is open, the guiding member of the open drawer will link the relative locking member to move the linking frame, which then links the other locking members. The guiding member of the open drawer triggers the relative locking member to lock the linking frame temporarily, and the other locking members block their relative guiding members so that the other drawers are unable to slide.