Invention Grant
- Patent Title: Engineered proteases for affinity purification and processing of fusion proteins
- Patent Title (中): 用于融合蛋白亲和纯化和加工的工程蛋白酶
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Application No.: US10567073Application Date: 2004-06-29
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Publication No.: US07824885B2Publication Date: 2010-11-02
- Inventor: Philip N. Bryan
- Applicant: Philip N. Bryan
- Applicant Address: US MD College Park
- Assignee: University of Maryland, College Park
- Current Assignee: University of Maryland, College Park
- Current Assignee Address: US MD College Park
- Agency: Intellectual Property/Technology Law
- Agent Kelly K. Reynolds; Steven J. Hultquist
- International Application: PCT/US2004/021049 WO 20040629
- International Announcement: WO2005/017110 WO 20050224
- Main IPC: C12N15/62
- IPC: C12N15/62 ; C12N15/70 ; C12N15/74 ; C12N15/75 ; C12N15/79 ; C12N15/81 ; C12N15/85 ; C12P21/06
Abstract:
The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.
Public/Granted literature
- US20060134740A1 Engineered proteases for affinity purification and processing of fusion proteins Public/Granted day:2006-06-22
Information query
IPC分类:
