Invention Grant
- Patent Title: Method of detecting nucleic acid by using nucleic acid microarray
- Patent Title (中): 使用核酸微阵列检测核酸的方法
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Application No.: US11815239Application Date: 2006-02-06
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Publication No.: US07824917B2Publication Date: 2010-11-02
- Inventor: Hiroyuki Ooshima
- Applicant: Hiroyuki Ooshima
- Applicant Address: JP Tokyo
- Assignee: Mitsubishi Rayon Co., Ltd.
- Current Assignee: Mitsubishi Rayon Co., Ltd.
- Current Assignee Address: JP Tokyo
- Agency: McDermott Will & Emery LLP
- Priority: JP2005-028843 20050204
- International Application: PCT/JP2006/302402 WO 20060206
- International Announcement: WO2006/083040 WO 20060810
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/00
Abstract:
The present invention provides a method of detecting a nucleic acid which is not restricted by the design of the base sequence of a nucleic acid probe. By repeating washing and detection in multiple stages, the present invention can improve the precision of sequence-specific hybridization stepwise and also can ease restrictions in designing the nucleic acid probes, in particular, restrictions on the Tm value (the temperature at which the nucleic acid double strand is dissociated into single strands) or the sequence length of the nucleic acid probes.
Public/Granted literature
- US20090011513A1 METHOD OF DETECTING NUCLEIC ACID BY USING NUCLEIC ACID MICROARRAY Public/Granted day:2009-01-08
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