A holographic storage and regeneration system includes a holographic recording medium, a light source, a spatial light modulator, and a conjugate servo light guidance portion. The light source generates a signal light and a reference light. The spatial light modulator modulates the incident signal light and makes it incident onto the medium along the incident direction of the signal light. The guidance portion guides a reference light to incident onto the medium in one direction and reflects it in another direction. A reference light and a signal light interfere with each other to produce a holographic interference fringe. The reflected reference light is guided into a first image sensor to be used to analyze the incident angle of the reference light. When the reference light incidents onto the fringe of the medium through the guidance portion in a reverse direction relative to another direction, a conjugate regenerated light is generated.