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发明授权
US07871779B2 Molecular identification of Aspergillus species 有权
曲霉菌的分子鉴定

Molecular identification of Aspergillus species
摘要：
Novel techniques for the detection of Aspergillus in samples are disclosed. These techniques relate to PCR amplification and/or detection of Aspergillus ITS1 rDNA sequences, and the identification of particular species of Aspergillus by detecting differences in the ITS1-V1, ITS-V2, ITS-V3, ITS-V4, and ITS-V5 nucleic acid sequences of Aspergillus. The highly variable regions of the ITS1 rDNA sequences are particularly useful in distinguishing, for example, Aspergillus clavatus, Aspergillus granulosus, Aspergillus sydowii, Aspergillus flavipes, Aspergillus restrictus, Aspergillus versicolor, Aspergillus wentii, and Aspergillus chevalieri. In particular embodiments, the sequence differences are also able to distinguish among variants of particular species, such as Aspergillus granulosus CBS 119.5A, Aspergillus granulosus strain NRRL 1932, Aspergillus sydowii strain NRRL 250, Aspergillus sydowii strain NRRL 4768, Aspergillus sydowii strain CUHI, Aspergillus sydowii strain CUH2, Aspergillus sydowii strain CUH7, and Aspergillus sydowii strain CUH8.
摘要（中）：

公开了用于检测样品中曲霉菌的新技术。 这些技术涉及通过检测ITS1-V1，ITS-V2，ITS-V3，ITS-V4和ITS-V5核酸中的差异来鉴定曲霉ITS1 rDNA序列的PCR扩增和/或检测，并鉴定特定的曲霉种 曲霉酸序列。 ITS1 rDNA序列的高度可变区特别可用于区分例如曲霉菌（Aspergillus clavatus），粒细菌（Aspergillus granulosus），曲霉（Aspergillus sydowii），曲霉（Aspergillus flavipes），限制曲霉（Aspergillus restrictionus），曲霉（Aspergillus versicolor），曲霉（Aspergillus goii）和曲霉（Aspergillus chevalieri）。 在特定的实施方案中，序列差异也能够区分特定种类的变体，例如粒细菌芽孢杆菌CBS 119.5A，粒细菌菌株NRRL 1932，曲霉菌株NRRL 250，曲霉菌菌株NRRL 4768，曲霉菌菌株CUHI，曲霉属（Aspergillus sydowii） sydowii菌株CUH2，曲霉菌菌株CUH7和曲霉菌菌株CUH8。

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