发明授权
- 专利标题： Gene encoding novel luciferase
- 专利标题（中）： 基因编码新型荧光素酶
-
申请号： US11721032申请日： 2004-12-09
-
公开(公告)号： US07871803B2公开(公告)日： 2011-01-18
- 发明人: Hiromi Takenaka
- 申请人： Hiromi Takenaka
- 申请人地址： JP Tokyo
- 专利权人： NEC Soft, Ltd.
- 当前专利权人： NEC Soft, Ltd.
- 当前专利权人地址： JP Tokyo
- 代理机构： Sughrue Mion, PLLC
- 国际申请： PCT/JP2004/018401 WO 20041209
- 国际公布： WO2006/061906 WO 20060615
- 主分类号： C12N15/53
- IPC分类号： C12N15/53 ; C12N9/02 ; C12Q1/68 ; C12Q1/66
摘要：
The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1. MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD60 LETDLFTIVE DMNVISRDTN LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC120 LSKIKCTAKM KVYIPGRCHD YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL180 CADCTTGCLK GLANVKCSAL LKKWLPDRCA SFADKIQSEV DNIKGLAGDR210
公开/授权文献
- US20090233320A1 GENE ENCODING NOVEL LUCIFERASE 公开/授权日：2009-09-17
信息查询
IPC分类:
