A method is provided for fabricating a semiconductor-on-insulator (“SOI”) substrate. In such method an SOI substrate is formed to include (i) an SOI layer of monocrystalline silicon separated from (ii) a bulk semiconductor layer by (iii) a buried oxide (“BOX”) layer including a layer of doped silicate glass. A sacrificial stressed layer is deposited onto the SOI substrate to overlie the SOI layer. Trenches are then etched through the sacrificial stressed layer and into the SOI layer. The SOI substrate is heated with the sacrificial stressed layer sufficiently to cause the glass layer to soften and the sacrificial stressed layer to relax, to thereby apply a stress to the SOI layer to form a stressed SOI layer. The trenches in the stressed SOI layer are then filled with a dielectric material to form trench isolation regions contacting peripheral edges of the stressed SOI layer, the trench isolation regions extending downwardly from a major surface of the stressed SOI layer towards the BOX layer. The sacrificial stressed layer is then removed to expose the stressed SOI layer. Field effect transistors can then be formed in the stressed SOI layer.