A fluid coupling assembly is disclosed comprising a connector body defining a bore extending axially forward from an entrance opening, a tubular male member with an upset, a seal member, and a retainer. The retainer is received within the bore and includes locking members to releaseably retain the tube. The retainer includes a forward extension to support the seal against fluid pressure. The retainer and seal are configured to be applied to the male member before insertion of the male member into the bore of the connector body. Two of the locking members include ramps extending radially outwardly to contact the body portion defining the entrance opening sequentially prior to contact by the locking arms of the other locking members. A removable protective cap attaches the retainer and seal onto a tube end and covers these components prior to assembly. The retainer, seal member and protective cap cannot be assembled onto the tube end unless the seal is present within the protective cap.