发明授权
- 专利标题： Protein expression in rodent cells
- 专利标题（中）： 啮齿动物细胞中的蛋白质表达
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申请号： US12083535申请日： 2006-10-26
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公开(公告)号： US07892790B2公开(公告)日： 2011-02-22
- 发明人: Ulrich Goepfert , Erhard Kopetzki
- 申请人： Ulrich Goepfert , Erhard Kopetzki
- 申请人地址： US NJ Nutley
- 专利权人： Hoffmann-La Roche Inc.
- 当前专利权人： Hoffmann-La Roche Inc.
- 当前专利权人地址： US NJ Nutley
- 代理商 George W. Johnston; Dennis P. Tramaloni
- 优先权： EP05023611 20051028
- 国际申请： PCT/EP2006/010308 WO 20061026
- 国际公布： WO2007/048601 WO 20070503
- 主分类号： C12P21/06
- IPC分类号： C12P21/06 ; C12N5/10
摘要：
A method is described for the expression of heterologous polypeptides in rodent cells. The method comprises the oriP/EBNA-1 episomal replication and maintenance system of the Epstein-Barr-Virus (EBV). With the stable integration of an EBNA-1-protein expression cassette under the control of a promoter into the genome of a rodent cell an EBNA-1-protein expression in the cells was obtained. The heterologous protein is expressed from an episome comprising an EBV origin of replication and a functional expression cassette of said heterologous protein. The invention further comprises transformed rodent cell lines, a method for the production of a heterologous protein in said cell lines and a kit for the construction of said cell lines.
公开/授权文献
- US20100021965A1 Protein Expression in Rodent Cells 公开/授权日：2010-01-28
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