发明授权
- 专利标题: Nucleic acid amplification with continuous flow emulsion
- 专利标题(中): 用连续流动乳液进行核酸扩增
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申请号: US11045678申请日: 2005-01-28
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公开(公告)号: US07927797B2公开(公告)日: 2011-04-19
- 发明人: John R. Nobile , William L. Lee , John H. Leamon
- 申请人: John R. Nobile , William L. Lee , John H. Leamon
- 申请人地址: US CT Branford
- 专利权人: 454 Life Sciences Corporation
- 当前专利权人: 454 Life Sciences Corporation
- 当前专利权人地址: US CT Branford
- 代理机构: Mintz Levin Cohn Ferris Glovsky and Popeop PC
- 代理商 Ivor R. Elrifi
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; F02M47/02
摘要:
Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.
公开/授权文献
- US20050227264A1 Nucleic acid amplification with continuous flow emulsion 公开/授权日:2005-10-13
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