发明授权
- 专利标题: Immunochromatography method using fragmented antibody
- 专利标题(中): 使用片段化抗体的免疫色谱法
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申请号: US12239640申请日: 2008-09-26
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公开(公告)号: US07935541B2公开(公告)日: 2011-05-03
- 发明人: Hiroyuki Chiku
- 申请人: Hiroyuki Chiku
- 申请人地址: JP Tokyo
- 专利权人: Fujifilm Corporation
- 当前专利权人: Fujifilm Corporation
- 当前专利权人地址: JP Tokyo
- 代理机构: Birch, Stewart, Kolasch & Birch, LLP
- 优先权: JP2007-254173 20070928; JP2008-029988 20080212
- 主分类号: G01N33/553
- IPC分类号: G01N33/553
摘要:
An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance modified with a first antibody against the analyte on a porous carrier in a state where the analyte and the labeling substance is mixed, and capturing the analyte and the labeling substance at the reaction site on the porous carrier having a second antibody against the analyte, so as to detect the analyte; wherein a fragmented antibody is used as the first antibody and/or the second antibody; an analyte is detected via amplification using an amplification solution which contains a compound containing silver and a reducing agent for silver ions; and the ratio of the non-specifically adsorbed labeling substance in the pre-amplification detection site and the non-detection site of the porous carrier is between 0.4 to 2.5 in a system where the density of the non-specifically adsorbed labeling substance is 106/mm2 or less.
公开/授权文献
- US20090087927A1 IMMUNOCHROMATOGRAPHY METHOD USING FRAGMENTED ANTIBODY 公开/授权日:2009-04-02
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