Invention Grant
- Patent Title: Thermostable Taq polymerase fragment
- Patent Title (中): 热稳定性Taq聚合酶片段
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Application No.: US10917157Application Date: 2004-08-12
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Publication No.: US07972830B2Publication Date: 2011-07-05
- Inventor: Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
- Applicant: Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
- Applicant Address: US IN Indianapolis
- Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee Address: US IN Indianapolis
- Priority: EP03017636 20030812
- Main IPC: C12N9/12
- IPC: C12N9/12
Abstract:
It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
Public/Granted literature
- US20050037412A1 Thermostable Taq polymerase fragment Public/Granted day:2005-02-17
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