A display substrate includes a base substrate, a gate line, a data line, a pixel electrode and a shielding electrode. The base substrate includes a plurality of unit pixel areas arranged in a matrix shape. The gate line extends between the unit pixel areas. The data line crosses the gate line. The data line extends between the unit pixel areas. The pixel electrode is disposed in the unit pixel area. The pixel electrode is electrically connected to an output electrode of a switching element electrically connected to the gate and data lines. The shielding electrode is disposed the gate and data lines. The shielding electrode has an opening an opening formed therein, the opening disposed above and extending along a direction of the gate line.