发明授权
- 专利标题: Cellulase variants
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申请号: US12394202申请日: 2009-02-27
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公开(公告)号: US07993898B2公开(公告)日: 2011-08-09
- 发明人: Kim Vilbour Andersen , Martin Schulein , Lars Christensen , Bo Damgaard , Claus Von der Osten
- 申请人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christensen , Bo Damgaard , Claus Von der Osten
- 申请人地址: DK Bagsvaerd
- 专利权人: Novozymes A/S
- 当前专利权人: Novozymes A/S
- 当前专利权人地址: DK Bagsvaerd
- 代理商 Elias Lambiris
- 优先权: DK1013/96 19960917
- 主分类号: C12N9/42
- IPC分类号: C12N9/42 ; C12N1/20 ; C12N15/00 ; C12Q1/00 ; C12Q1/68 ; C12P21/04 ; C07H21/04
摘要:
The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
公开/授权文献
- US20090170747A1 CELLULASE VARIANTS 公开/授权日:2009-07-02