In one embodiment, a male coupling connectable to and separable from a female coupling (e.g., a female threaded port) is provided. The female coupling includes a receiving portion sized to receive at least a portion of the male coupling, where the receiving portion has internal threads provided therein. The male coupling includes a body having a passageway extending therethrough and a locking member disposed about the body and configured to move between locking and releasing positions. The locking member includes a retaining formation configured to engage the internal threads of the female coupling when the locking member is in its locking position. The male coupling further includes a locking sleeve disposed about and axially movable with respect to the body between first and second positions. After insertion of the male coupling into the female coupling and upon movement of the locking sleeve to its first position, the locking sleeve forces the locking member to move to its locking position causing the retaining formation of the locking member to engage the internal threads of the female coupling, thereby locking the male coupling and the female coupling together.