发明授权
- 专利标题: Papillomavirus vaccines
- 专利标题(中): 乳头瘤病毒疫苗
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申请号: US08764926申请日: 1996-12-16
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公开(公告)号: US08012679B1公开(公告)日: 2011-09-06
- 发明人: C. Richard Schlegel , A. Bennett Jenson
- 申请人: C. Richard Schlegel , A. Bennett Jenson
- 申请人地址: US DC Washington
- 专利权人: Georgetown University
- 当前专利权人: Georgetown University
- 当前专利权人地址: US DC Washington
- 代理机构: Carella, Byrne et al.
- 代理商 Elliot M. Olstein; Raymond J. Lillie
- 主分类号: C12Q1/70
- IPC分类号: C12Q1/70
摘要:
Seven polyclonal and monoclonal antibodies were characterized for their ability to react specifically with either conformational or non-conformational epitopes of the HPV-1 virion. Using these antibodies, it was shown that the HPV-1 L1 protein (when expressed by an SV40 vector in cos cells) displayed conformational epitopes characteristic of intact viral particles. In addition, the L1 capsid protein was translocated normally into cell nuclei, was of appropriate size (57kD), and could be isolated in native form by immunoprecipitation techniques. Most importantly, the screening of expressed papillomavirus capsid proteins for reactivity with conformation-dependent antibodies represents a new, general methodology for ensuring that such proteins will be suitable for use in vaccine development or in the serologic detection/typing of human papillomavirus infections.