In a display substrate and a display device having the display substrate for reducing a line resistance, the display substrate includes a plurality of pixel parts, a signal line part and a signal pad part. The pixel parts are formed in a display area. The signal line part is formed in a peripheral area surrounding the display area. The signal line part includes a first line formed with a first conductive layer, and a second line formed with a second conductive layer over the first line. The second line overlaps the first line. The signal pad part includes a first pad integrally formed with the first line, and a second pad integrally formed with the second line, wherein an identical driving signal is applied to the first pad and the second pad. Accordingly, the line resistance of the signal line part may be reduced.