The present invention relates to image rejection calibration in a radio frequency (RF) receiver. In one embodiment, the receiver includes a receiver front-end and an image rejection calibration system. The image rejection calibration system provides a first test signal to an RF input of the receiver front-end, and obtains first measurements of a quadrature output signal output from the receiver front-end while the first test signal is applied to the RF input. The image rejection calibration system then applies a second test signal to the RF input of the receiver front-end that is approximately 90 degrees out-of-phase with the first test signal, and obtains second measurements of the quadrature output signal while the second test signal is applied to the RF input. Based on the first and second measurements, the image rejection calibration system determines an amplitude error and a phase error of the receiver front-end.