发明授权
US08093060B2 Multisite phosphorylated peptide (protein) recognizing compound and detection method, imaging method, alzheimer's disease diagnosing method and reagent kit using the same
有权
多位点磷酸化肽(蛋白质)识别化合物和检测方法,成像方法,阿尔茨海默病诊断方法和使用该方法的试剂盒
- 专利标题: Multisite phosphorylated peptide (protein) recognizing compound and detection method, imaging method, alzheimer's disease diagnosing method and reagent kit using the same
- 专利标题(中): 多位点磷酸化肽(蛋白质)识别化合物和检测方法,成像方法,阿尔茨海默病诊断方法和使用该方法的试剂盒
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申请号: US12393011申请日: 2009-02-25
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公开(公告)号: US08093060B2公开(公告)日: 2012-01-10
- 发明人: Itaru Hamachi , Fumio Yamauchi , Tetsuya Yano , Kimihiro Yoshimura , Akio Ojida , Takashi Sakamoto , Masaaki Inoue
- 申请人: Itaru Hamachi , Fumio Yamauchi , Tetsuya Yano , Kimihiro Yoshimura , Akio Ojida , Takashi Sakamoto , Masaaki Inoue
- 申请人地址: JP Tokyo
- 专利权人: Canon Kabushiki Kaisha
- 当前专利权人: Canon Kabushiki Kaisha
- 当前专利权人地址: JP Tokyo
- 代理机构: Fitzpatrick, Cella, Harper & Scinto
- 优先权: JP2008-048281 20080228
- 主分类号: G01N33/48
- IPC分类号: G01N33/48
摘要:
A compound which captures a multisite phosphorylated peptide or protein specifically to a phosphorylation site and a method for detecting the peptide or protein using the compound. Particularly, a compound which specifically detects an excessively phosphorylated tau protein observed in the brain affected by Alzheimer's disease and a method for diagnosing Alzheimer's disease in vitro or in vivo using the compound are provided. By bringing a metal complex compound having two dipicolylamine (Dpa) moieties and a spacer including a chromogenic or luminescent functional or atom group into contact with a multisite phosphorylated peptide or protein, the compound recognizes the distance between phosphate groups and specifically binds to the peptide or protein, and a multisite phosphorylated peptide or protein or kinase activity is optically detected by measuring the change, or a multisite phosphorylated peptide or protein or kinase activity is imaged by an optical imaging method applying the change in luminescence.
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