A data line driving method adapted in a display panel driver circuit is provided. The display panel driver circuit comprises a plurality rows of gate lines and a plurality of data driver stages each corresponding to a data line group, wherein the data line driving method comprises the steps of: turning on the data driver stages in a first sequential order to input a first frame data in the data line groups corresponding to the data driver stages in each gate line activation time within a first frame period; and turning on the data driver stages in a second sequential order which is opposed to the first sequential order to input a second frame data in the data line groups corresponding to the data driver stages in each gate line activation time within a second frame period; wherein the first and the second frame period are two interlaced periods next to each other.