An anti-virus method based on a security chip according to the present invention is provided. The method comprises the following steps: a hash value obtained by a hashing operation for a computer key file and a system control program are stored in a memory of the security chip, and a backup file of the computer key file is stored in a backup storage area. When power up, the integrity of the system control program is verified by using the hash value of the system control program stored in the memory of the security chip. If the system control program is integral, a control is executed by the system control program, and the system control program verifies the integrity of the computer key file using the hash value of the computer key file stored in the memory of the security chip. If all the computer key files are integral, the operating system is started; on the contrary, if any of the computer key file is not integral, it will be restored using the backup file of the computer key file stored in the backup storage area.