An optical transmission system for performing frequency synchronization even with a client signal with low frequency accuracy, and for transmitting thereof by accommodating/multiplexing without causing a bit slip. A new overhead is added to the entire client signal, and the signal including the new overhead being stuffed is transmitted in conjunction with a plurality of stuffing bits as an optical signal wherein a data storing bit for a negative stuffing, a stuffing information notification bit, and a stuff bits inserting bit for a positive stuffing in the payload are defined in plurality as stuffing bits for adjusting clock frequencies of the client signal in this new overhead.