An assembly fixture for fixing a part/component to a printed circuit board includes a platform, an elevating unit, and a carrying body. The platform has a receiving area and a plurality of positioning pillars. The elevating unit is disposed on the platform. The carrying body is coupled with the elevating unit, configured to correspond in position to the receiving area, configured to carry the printed circuit board, and provided therein with a plurality of through holes corresponding in position to the positioning pillars, respectively. The elevating unit is capable of descending to enable the positioning pillars to protrude from the printed circuit board and the through holes, respectively, for positioning the part/component on the printed circuit board. The assembly fixture is effective in positioning the part/component on the printed circuit board to render an assembly process of the printed circuit board easy, quick, safe, and precise.