An active matrix substrate including a substrate, a plurality of scan lines, a plurality of data lines and a plurality of sub-pixels is provided. The scan lines and the data lines are disposed on the substrate, and define a plurality of sub-pixel regions distributed in a delta arrangement. The sub-pixels corresponding to the sub-pixel regions are disposed on the substrate. The sub-pixels are electrically connected with corresponding scan lines and corresponding data lines. Between two sub-pixel regions corresponding to any two adjacent sub-pixels at a same side of one scan line, there are two data lines. Each sub-pixel includes an active device and a pixel electrode. The active device is electrically connected with a corresponding scan line and a corresponding data line. The pixel electrode is electrically connected with the active device, and extends from the sub-pixel region corresponding to the sub-pixel to a position over the data line.