发明授权
- 专利标题： Optimized purification process of recombinant growth factor protein
- 专利标题（中）： 优化重组生长因子蛋白纯化过程
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申请号： US12670734申请日： 2008-06-20
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公开(公告)号： US08187837B2公开(公告)日： 2012-05-29
- 发明人: Ute Ehringer , Eva Kohlstrung
- 申请人： Ute Ehringer , Eva Kohlstrung
- 申请人地址： DE Heidelberg
- 专利权人： Biopharm Gesellschaft zur biotechnologischen Entwicklung von Pharmaka mbH
- 当前专利权人： Biopharm Gesellschaft zur biotechnologischen Entwicklung von Pharmaka mbH
- 当前专利权人地址： DE Heidelberg
- 代理机构： Rothwell, Figg, Ernst & Manbeck, P.C.
- 优先权： EP07014798 20070727
- 国际申请： PCT/EP2008/005016 WO 20080620
- 国际公布： WO2009/015736 WO 20090205
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12N1/06 ; C12N1/21 ; C07K14/51
摘要：
A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.
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