发明授权
- 专利标题： Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
- 专利标题（中）： 使用光收获多色素探测和分析多核苷酸的方法和组合物
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申请号： US12632734申请日： 2009-12-07
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公开(公告)号： US08227187B2公开(公告)日： 2012-07-24
- 发明人: Guillermo C. Bazan , Brent S. Gaylord , Shu Wang
- 申请人： Guillermo C. Bazan , Brent S. Gaylord , Shu Wang
- 申请人地址： US CA Oakland
- 专利权人： The Regents of the University of California
- 当前专利权人： The Regents of the University of California
- 当前专利权人地址： US CA Oakland
- 代理机构： TechLaw LLP
- 代理商 Sam K. Tahmassebi
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/00 ; C07H21/02 ; C07H21/04 ; C07H19/04
摘要：
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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