Apparatus, method and system for delivering sterile unit dose of ozone. System includes sterile vial containing oxygen and corona discharge assembly. Sterile vial is engaged to ozone conversion unit having high voltage transformer, ozone measurement mechanism, data input mechanism, and displayed read-out. When power is supplied to sterile vial containing corona discharge assembly, oxygen is converted to specified concentration of ozone. Sterile vial is disengaged from ozone conversion unit, drawing port is attached, and needle or similar extraction tool is used to withdrawal ozone for treatment.