Quality settings established by an encoder are adjusted based on information associated with regions of interest (“ROIs”). For example, quantization step sizes can be reduced (to improve quality) or increased (to reduce bit rate). ROIs can be identified and quality settings can be adjusted based on input received from a user interface. An overlap setting can be determined for a portion of a picture that corresponds to an ROI overlap area. For example, an overlap setting is chosen from step sizes corresponding to a first overlapping ROI and a second overlapping ROI, or from relative reductions in step size corresponding to the first ROI and the second ROI. ROIs can be parameterized by information (e.g., using data structures) that indicates spatial dimensions of the ROIs and quality adjustment information (e.g., dead zone information, step size information, and quantization mode information).