A biologic and medical multi-channel low voltage micro-electric-field generator including a power supply unit, at least one micro-electric-field generating unit set comprising a step-down unit, a linear regulator unit, and a pulse generating and outputting unit, and a programmable logic control unit. The micro-electric-field generating unit set is connected to an output end of the power supply unit. The step-down unit depresses the voltage of frequency power. The linear regulator unit regulates the output of the step-down unit. The pulse generating and outputting unit turns on/off the output of the linear regulator unit and connects to a network electrode group. The programmable logic control unit controls the characteristics of the output pulse from the pulse generating and outputting unit. The generator is applicable in gene, protein, drug and/or a variety of plasmids delivery to the organs, cells within the tissues of large animal or human.