An optimization method of multiple service flows operation for a WiMAX system includes: adding a resource combination indication to a hand-off request message, a path pre-registration request message and a path registration request message; hand-off request processing: when multiple service flows are handed off by a mobile station from a current serving network to a target network, determining whether the mobile station is allowed to hand off to a target network according to the resource combination indication in the hand-off request message and whether the handoff of the multiple service flows is accepted by the target network; path pre-establishment processing and path establishment processing: when the mobile station is allowed to hand off multiple service flows from the current serving network to the target network, determining whether data paths between the target network and the network where a data management unit is located can be pre-established/established according to the resource combination indication in the path pre-registration request message and the path registration request message and whether the data path pre-registration/registration of the multiple service flows can be established.