发明授权
US08268581B2 Carboxy terminal residues as predictors and regulators of protein stability
有权
羧基末端残基作为蛋白质稳定性的预测因子和调节物
- 专利标题: Carboxy terminal residues as predictors and regulators of protein stability
- 专利标题(中): 羧基末端残基作为蛋白质稳定性的预测因子和调节物
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申请号: US12431315申请日: 2009-04-28
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公开(公告)号: US08268581B2公开(公告)日: 2012-09-18
- 发明人: Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
- 申请人: Brian G. Fox , Yong Chang , Gary A. Wesenberg , Craig A. Bingman
- 申请人地址: US WI Madison
- 专利权人: Wisconsin Alumni Research Foundation
- 当前专利权人: Wisconsin Alumni Research Foundation
- 当前专利权人地址: US WI Madison
- 代理机构: Quarles & Brady LLP
- 主分类号: C12P21/06
- IPC分类号: C12P21/06 ; C07K14/00 ; C07H21/04
摘要:
Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in Mycobacterium smegmatis. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.