A display substrate includes a driving element, a switching element, a gate line, a data line, a driving voltage line and an electroluminescent element. The driving element includes a driving control electrode formed from a first conductive layer, and a driving input electrode and a driving output electrode formed from a second conductive layer. The switching element includes a switching control electrode formed from the second conductive layer, and a switching input electrode and a switching output electrode formed from a third conductive layer. The gate and data lines are formed from the second and third conductive layers, respectively. The driving voltage line is formed from the third conductive layer. Thus, misalignment between upper and lower patterns may be prevented to improve the reliability of a manufacturing process and increase an aperture ratio, thereby enhancing display quality.