The present invention provides a rubber composition capable of reducing rolling resistance, and improving abrasion resistance, wet skid performance, and steering stability. A tire having a tread and/or a sidewall using the rubber composition is also provided. The rubber composition of the present invention comprises not less than 10 parts by weight of the silica (1) having an average primary particle diameter of not less than 22 nm and not less than 5 parts by weight of the silica (2) having an average primary particle diameter smaller than 22 nm based on 100 parts by weight of a rubber component, wherein the total amount of the silica (1) and the silica (2) is 15 to 150 parts by weight. The tire of the present invention is prepared by using the rubber composition.