A light-emitting adjustment method and a display device are provided. The display device includes a voltage source, a light-emitting diode array, a pulse width modulator, a current sensor and a light-emitting adjuster. The voltage source provides an operating voltage. The pulse width modulator provides operating pulse signals to multiple light-emitting diodes arranged in column in order. The current sensor senses a plurality of overall current values of the light-emitting diodes at different timings during the light-emitting diodes are sequentially enabled. The light-emitting adjuster computes an operating current value of each of the light-emitting diodes according to the overall current values and performs a compensation operation based on the operating current value to obtain and output a compensation signal.