Disclosed are a color substrate, a manufacturing method thereof and a liquid crystal display. The color substrate comprises a substrate; black matrices disposed on the substrate; color pixels whose material is fluorescent material disposed alternately among the black matrices; a flat encapsulation layer covering the black matrices and the color pixel; and a built-in polarizer attached to the flat encapsulation layer. The manufacturing method comprises forming black matrices on a substrate, and forming color pixels of fluorescent material among the black matrices; form a flat encapsulation layer; and form a built-in polarizer. The liquid crystal display by using the color substrate of the present invention further comprises a array substrate, a liquid crystal layer and a back light wherein wavelength of light emitted by the back light is shorter than of equal to stimulated luminescence critical wavelength of fluorescent material of the color pixels on the color substrate. In the present invention, the color fluorescent material is used for replacing the color resin material on the color substrate, the lamination mode is changed and the energy loss resulting from light-absorbing of the resin material is decreased, which could increase the light utilization efficiency and improve the visual angle characteristic of the liquid crystal display.