Roll-to-roll printing apparatuses are disclosed. The roll-to-roll printing apparatus includes a printing device with a squeegee moving along a first direction transferring a pattern of a stencil to a roll of working pieces. A sending roller and a receiving roller are disposed on both sides of the printing device. The roll of working pieces is rolled along a second direction to a printing stage of the printing device. An alignment device is disposed corresponding to a position of the stencil and the roll of working pieces. A drying device is disposed on one side of the printing device and may be on the same side with the receiving roller, wherein the drying device cures the patterns on the roll of working pieces.