A telescoping floor seal assembly comprising an attachment portion adapted to be mounted to a bottom edge of a vertically displaceable partition, and a displaceable sealing portion slideably mounted within the attachment portion for relative displacement therewith between a fully collapsed position and a fully extended position and described. The displaceable sealing portion has at least one longitudinal sealing edge member at a base thereof for contacting a floor when the vertical partition is displaced to a closed position. The attachment portion is substantially vertically displaceable with the vertically displaceable partition while the sealing edge member of the displaceable sealing portion remains in sealing contact with the floor within a predetermined displacement range of vertical movement of the vertical partition. The telescoping floor seal assembly thereby accommodates live load deflections of the vertically displaceable partition without compromising the seal formed between the floor and the telescoping floor seal.