发明授权
- 专利标题： Polymerase enzymes and reagents for enhanced nucleic acid sequencing
- 专利标题（中）： 用于增强核酸测序的聚合酶和试剂
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申请号： US11977160申请日： 2007-10-22
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公开(公告)号： US08343746B2公开(公告)日： 2013-01-01
- 发明人: David R. Rank , Arek Bibillo , Paul Peluso
- 申请人： David R. Rank , Arek Bibillo , Paul Peluso
- 申请人地址： US CA Menlo Park
- 专利权人： Pacific Biosciences of California, Inc.
- 当前专利权人： Pacific Biosciences of California, Inc.
- 当前专利权人地址： US CA Menlo Park
- 代理机构： Quine Intellectual Property Law Group, P.C.
- 代理商 Monica Elrod-Erickson; Robert Reamey
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12P19/34
摘要：
Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant Φ29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including α-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.